嗜酸性粒细胞阳离子型核糖核酸酶4/嗜酸性粒细胞源神经**抗体
规格：1mg/1ml
英文名： RNASE2
别名： RNASE2; EDN; RNS2; RNAS2_HUMAN; Non-secretory ribonuclease; Eosinophil-derived neurotoxin;; RNase UpI-2; Ribonuclease 2; RNase 2; Ribonuclease US; Ear4; EC=3.1.27.5; EDN; Eosinophil cationic type ribo
分子量： 14kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human RNASE2
交叉反应：Human, Mouse, Rat,
细胞定位：细胞浆
嗜酸性粒细胞阳离子型核糖核酸酶4/嗜酸性粒细胞源神经**抗体产品介绍：background: Human and animal eosinophils contain a powerful neurotoxin that causes selective neuronal and axonal damage to white matter of cerebellum and spinal cord of experimental animals when injected intrathecally. This reaction is termed the "Gordon phenomenon." We purified the eosinophil-derived neurotoxin from eosinophil-rich leukocyte suspensions or eosinophil granules from four patients with various hypereosinophilic syndromes. A single protein with an average molecular weight of 18,400 was isolated by sequential chromatography on Sephadex G-50 columns and analyzed by sodium dodecyl 嗜酸性粒细胞阳离子型核糖核酸酶4/嗜酸性粒细胞源神经**抗体sulfate/polyacrylamide gel electrophoresis of column fractions. The purified eosinophil-derived neurotoxin from the cells of these patients retained the potent neurotoxic activity of the crude eosinophil or eosinophil granule extracts in experimental animals. These animals developed the syndrome of stiffness and ataxia progressing to severe paralysis characteristic of the Gordon phenomenon. Histologic examination of the brains of animals injected with purified eosinophil-derived neurotoxin confirmed the characteristic widespread loss of Purkinje cells and severe spongiform vacuolation in the white matter of cerebellum, brain stem, and spinal cord. We have established the location of eosinophil-derived neurotoxin in the eosinophil granule and have shown that it is distinct from several other eosinophil proteins, the granule major basic protein, and the Charcot-Leyden crystal protein (lysophospholipase). Subcellular Location: Lysosome (Probable). Cytoplasmic granule. Matrix of 嗜酸性粒细胞阳离子型核糖核酸酶4/嗜酸性粒细胞源神经**抗体eosinophil's large specific granule. Similarity: Belongs to the pancreatic ribonuclease family. Gene ID: 6036 Database links: Entrez Gene: 6036 Human Omim: 131410 Human SwissProt: P10153 Human Unigene: 728 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
嗜酸性粒细胞阳离子型核糖核酸酶4/嗜酸性粒细胞源神经**抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 **学
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid