神经***颗粒蛋白5抗体
规格：1mg/1ml
英文名： Secretogranin V
别名： 7B2; 7B2 protein; APPG; Neuroendocrine protein 7B2; P7B2; Pituitary polypeptide; Pituitary polypeptide; Prohormone convertase chaperone; SCG 5; SCG5; Secretogranin 5; Secretogranin V 7B2 protein; Secr
分子量： 17/21kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human PHF21A
交叉反应：Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep,
细胞定位：分泌型蛋白
神经***颗粒蛋白5抗体产品介绍：background: Sg V is a 212 amino acid protein that is secreted by endocrine and neuroendocrine secretory granules and belongs to the 7B2 family. Existing as two alternatively spliced isoforms, Sg V interacts with PC2 and, via this interaction, functions as a molecular chaperone for PC2, effectively preventing its premature activation in regulated secretory pathways. More specifically, Sg V binds to PC2 and facilitates its transport from the endoplasmic reticulum to secretory compartments, thus allowing PC2 to be cleaved and activated during the correct phase of the regulated secretory pathway. Sg V is subject to post-translational sulfation on specific tyrosine residues and is underexpressed in medulloblastomas, suggesting a role in tumor suppression. The gene encoding Sg V maps to human chromosome 15, which houses over 700 genes and comprises nearly 3% of the human genome. Function: Secretogranin V may have intracellular functions other than PC2 maturation in certain cells, although some recent work shows a discordancy between mRNA expression and enzymic activity. 神经***颗粒蛋白5抗体The mechanism for PC2 maturation appears to involve pro-Secretogranin V binding to inactive pro-PC2 via its polyproline motif (116 PPNPCP 121) in the endoplasmic reticulum and chaperoning pro-PC2 to a later secretory pathway compartment where maturation of PC2 proceeds once pro-Secretogranin V is proteolytically processed by a furin-like convertase into a 21kDa fragment and a C-terminal peptide. It is the C-terminal peptide that potently inhibits maturation of pro-PC2 until the complex is subjected to a decreasing pH gradient along the secretory pathway. It seems likely that mature PC2 then cleaves the CT peptide at its internal lys-lys site. Secretogranin V knockout mice develop a severe Cushings-like phenotype and exhibit multiple metabolic and hormonal abnormalities, indicating that Secretogranin V is required for activation of PC2 in vivo. Conversely, PC2-null mice appear to be viable. Subunit: Interacts with PCSK2/PC2 early in the secretory pathway. Dissociation occurs at later stages. Subcellular神经***颗粒蛋白5抗体 Location: Secreted. Note=Neuroendocrine and endocrine secretory granules. Tissue Specificity: Highly expressed in brain. Expressed at much lower level in other tissues. Post-translational modifications: Proteolytically cleaved in the Golgi by a furin-like convertase to generate bioactive peptides. Sulfated on tyrosine residues. Similarity: Belongs to the 7B2 family. Gene ID: 6447 Database links: Entrez Gene: 6447 Human Entrez Gene: 20394 Mouse Entrez Gene: 397110 Pig Entrez Gene: 25719 Rat Omim: 173120 Human SwissProt: P05408 Human SwissProt: P12961 Mouse SwissProt: P01165 Pig SwissProt: P27682 Rat Unigene: 156540 Human Unigene: 4836 Mouse Unigene: 6173 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
神经***颗粒蛋白5抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 神经生物学 信号转导 锌指蛋白
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid