磷酸化酪氨酸蛋白激酶受体B1+B2抗体
规格：1mg/1ml
英文名： phospho-EphB1+EphB2 (Tyr594)
别名： Eph receptor B1 + Eph receptor B2 (phospho Y594); p-Eph receptor B1 + Eph receptor B2 (phospho Y594); Eph receptor B1+Eph receptor B2; EPH B1; EPH B2; Eph tyrosine kinase 2; Eph tyrosine kinase 3; EPH
分子量： 110kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthesised phosphopeptide derived from human
交叉反应：Human, Mouse, Rat,
细胞定位：细胞膜
磷酸化酪氨酸蛋白激酶受体B1+B2抗体产品介绍：background: Ephrin receptors bind members of the ephrin ligand family. They are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. They make up the largest subgroup of the receptor tyrosine kinase (RTK) family. Eph receptor B1 (EphB1) and Eph receptor B2 (EphB2) are receptors for ephrin-B family members. Developing and ***** neural tissue express nearly all of the Ephrin receptors and ephrin ligands. Ephrins and ephrin receptors also play a significant role in angiogenesis. EphB2 acts as a tumor suppressor. Function: Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Cognate/functional ephrin ligands for this receptor include EFNB1, EFNB2 and EFNB3. During nervous磷酸化酪氨酸蛋白激酶受体B1+B2抗体 system development, regulates retinal axon guidance redirecting ipsilaterally ventrotemporal retinal ganglion cells axons at the optic chiasm midline. This probably requires repulsive interaction with EFNB2. In the ***** nervous system together with EFNB3, regulates chemotaxis, proliferation and polarity of the hippocampus neural progenitors. Beside its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and synapse formation. May also regulate angiogenesis. More generally, may play a role in targeted cell migration and adhesion. Upon activation by EFNB1 and probably other ephrin-B ligands activates the MAPK/ERK and the JNK signaling cascades to regulate cell migration and adhesion respectively. Subunit: Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses. Interacts with EPHB6; transphosphorylates EPHB6 to form an active signaling complex. Interacts with PICK1. Interacts (through Tyr-594) with NCK1 (via SH2 domain); activates the JUN cascade to regulate cell adhesion. The ligand-activated form interacts (through Tyr-928) with GRB7 and GRB10 (via SH2 domains). The ligand-activated form interacts (residues within the catalytic domain) with GRB2 (via SH2 domain). Interacts with GRB2, SHC1 and SRC; activates the MAPK/ERK cascade to regulate cell migration. Interacts with CBL; regulates receptor degradation through ubiquitination. Interacts with ACP1. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Early endosome membrane. Cell projection, dendrite. Tissue Specificity: Preferentially expressed in brain. 磷酸化酪氨酸蛋白激酶受体B1+B2抗体Post-translational modifications: Phosphorylated. Autophosphorylation is stimulated by the ligand EFNB1. Required for interaction with SH2 domain-containing interactors, for activation of the MAPK/ERK and JUN signaling cascades and for ubiquitination by CBL. Ubiquitinated; (EFNB1)ligand-induced poly- and/or multi-ubiquitination by CBL is regulated by SRC and leads to lysosomal degradation. Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily. Contains 1 Eph LBD (Eph ligand-binding) domain. Contains 2 fibronectin type-III domains. Contains 1 protein kinase domain. Contains 1 SAM (sterile alpha motif) domain. Gene ID: 2047 Database links: Entrez Gene: 2047 Human Entrez Gene: 270190 Mouse Entrez Gene: 24338 Rat Omim: 600600 Human SwissProt: P54762 Human SwissProt: Q8CBF3 Mouse SwissProt: P09759 Rat Unigene: 116092 Human Unigene: 22897 Mouse Unigene: 46606 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
磷酸化酪氨酸蛋白激酶受体B1+B2抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：心血管 细胞生物 神经生物学 信号转导 激酶和磷酸酶 细胞膜受体
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid