丝氨酸/苏氨酸蛋白激酶PLK1
规格：1mg/1ml
英文名： PLK1
别名： Cell cycle regulated protein kinase; PLK 1; PLK; PLK-1; plk1; PLK1_XENLA; polio like kinase; Polo like kinase 1; Polo-like kinase 1; Serine/threonine protein kinase; Serine/threonine protein kinase 13
分子量： 68kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from Xenopus laevis
交叉反应：Xenopus laevis
细胞定位：细胞核 细胞浆
丝氨酸/苏氨酸蛋白激酶PLK1产品介绍：background: Polo-like kinase (PLK) plays an important role in cell cycle regulation. It is required for bipolar spindle formation, activation of the anaphase-promoting complex/cyclosome, and cytokinesis. Recent work led to the identification of a polo-like kinase kinase (PLKK) that is thought to be responsible for activation of PLK. Recent work (Erikson, et al., 2004) has shown that PLKK is in turn activated by phosphorylation at three sites (Ser482, Ser486 and Ser490). Thus activation of PLK is thought to involve a kinase cascade involving the phosphorylation of Ser482,486,490 in PLKK. Function: Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of anaphase-promoting complex/cyclosome (APC/C) inhibitors, and the regulation of mitotic exit and cytokinesis. 丝氨酸/苏氨酸蛋白激酶PLK1Polo-like kinase proteins acts by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates cdc25, pkmyt1/myt1, stag2/sa2, tpx2. Plays multiple essential roles during mitosis. Phosphorylates the N-terminal domain of cdc25, which leads to cyclin b-cdc2 activation and mitotic entry. Also required for organization of bipolar spindles, and for exit from mitosis. Involved in kinetochore functions and sister chromatid cohesion by phosphorylating stag2/sa2. Subunit: Interacts with plk1 and kif2a. Subcellular Location: Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle.丝氨酸/苏氨酸蛋白激酶PLK1 Midbody. Note=Localized at centrosomes at prophase, spindles at metaphase, and at the midbody during cytokinesis. Tissue Specificity: Placenta and colon. Post-translational modifications: Activated by phosphorylation on Thr-201 during M phase. Phosphorylated by stk10, leading to activation during oocyte maturation. Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) in anaphase and following DNA damage, leading to its degradation by the proteasome. Protein levels are down-regulated by proteasomal degradation in anaphase. Similarity: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 2 POLO box domains. Contains 1 protein kinase domain. Gene ID: 380481 Database links: Entrez Gene: 380481 Xenopus laevis SwissProt: P70032 Xenopus laevis Unigene: 28141 Xenopus laevis Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
丝氨酸/苏氨酸蛋白激酶PLK1产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid