γ谷氨酰半胱氨酸合成酶抗体
规格：1mg/1ml
英文名： GCLC
别名： Gamma ECS; Gamma GCS; Gamma glutamylcysteine synthetase; Gamma-ECS; Gamma-glutamylcysteine synthetase; GCL; GCLC; GCLM; GCS; GCS Heavy Chain antibody GLCL; GLCLC; GLCLR; Glutamate cysteine ligase cata
分子量： 73kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human GCLC
交叉反应：Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit,
细胞定位：细胞浆
γ谷氨酰半胱氨酸合成酶抗体产品介绍：background: The GCLC gene consists of 16 exons and encodes the 636 amino acid protein g-GCSc (g-glutamylcysteine synthetase heavy subunit), also designated g-L-glutamate-L-cysteine ligase catalytic subunit (GLCLC). g-GCSc is expressed in hemocytes, brain, liver and kidney. g-GCSc associates with a regulatory or modifier subunit, g-GCSm (g-glutamylcysteine synthetase light subunit), to form a heterodimer, g-GCS. g-GCS is the first enzyme involved and the rate determining step in glutathione biosynthesis. Oxidants, cadium and methyl mercury upregulate the transcription of g-GCS. H2O2 regulation depends on the Yap1 protein and the presence of glutamate, glutamine and lysine. Cadium regulates transcription through proteins Met-4, Met-31 and Met-32. Cbf1, a DNA binding protein, inhibits transcription of g-GCS. Chemopreventive compounds cause increased levels of g-GCSc in kidney tissues, which may protect against γ谷氨酰半胱氨酸合成酶抗体chemically induced carcinogenesis. A His370Leu amino acid change in g-GCSc causes deficiencies in activity which are responsible for hemolytic anemia and low red blood cell glutathione levels. Defects in GCLC are the cause of hemolytic anemia. Subunit: Heterodimer of a catalytic heavy chain and a regulatory light chain. DISEASE: Defects in GCLC are the cause of hemolytic anemia (HAGGSD) [MIM:230450]. Similarity: Belongs to the glutamate--cysteine ligase type 3 family. Database links: Entrez Gene: 2729 Human Entrez Gene: 14629 γ谷氨酰半胱氨酸合成酶抗体Mouse Entrez Gene: 25283 Rat Omim: 606857 Human SwissProt: P48506 Human SwissProt: P97494 Mouse SwissProt: P19468 Rat Unigene: 654465 Human Unigene: 89888 Mouse Unigene: 8365 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产γ谷氨酰半胱氨酸合成酶抗体品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：肿瘤 细胞生物 **学 神经生物学 信号转导
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid