泛素结合酶E2蛋白A抗体
规格：1mg/1ml
英文名： UBE2A
别名： BHR6A; hHR6A; HR6A; mHR6A; RAD6 homolog A; RAD6A; RAD6B; UBC-1; UBC2; UBC6; UBCD6; UBE2A; UBE2A_HUMAN; UBE2B; Ubiquitin carrier protein A; Ubiquitin carrier protein; Ubiquitin conjugating enzyme E2 17
分子量： 17kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human UBE2A/UB
交叉反应：Human, Mouse, Rat, Chicken, Cow,
细胞定位：
泛素结合酶E2蛋白A抗体产品介绍：background: The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. The first step requires the ATP-dependent activation of the Ub C-terminus and the assembly of multi-Ub chains by the Ub-activating enzyme known as the E1 component. The Ub chain is then conjugated to the Ub-conjugating enzyme (E2) to generate an intermediate Ub-E2 complex. The Ub-ligase (E3) then catalyzes the transfer of Ub from E2 to the appropriate protein substrate. UBE2A (Ubiquitin-conjugating enzyme E2 A) and UBE2B (Ubiquitin-conjugating enzyme E2 B) are both Ub-conjugating enzymes that are essential to postreplication repair of UV-damaged DNA. UBE2A and UBE2B are both nuclear and cell membrane proteins that have been found to interact with Rad18. Function: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination 泛素结合酶E2蛋白A抗体of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11', as well as 'Lys-48'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Subunit: Interacts with RAD18 and WAC. DISEASE: Defects in UBE2A are the cause of mental retardation syndromic X-linked Nascimento-type (MRXSN) [MIM:300860]. Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRXSN features include dysmorphic facies, hirsutism, skin and nails abnormalities, obesity, speech anomalies and seizures. Similarity: Belongs to the ubiquitin-泛素结合酶E2蛋白A抗体conjugating enzyme family. Gene ID: 7319 Database links: Entrez Gene: 395672 Chicken Entrez Gene: 282107 Cow Entrez Gene: 7319 Human Entrez Gene: 22209 Mouse Entrez Gene: 298317 Rat Entrez Gene: 398788 Xenopus laevis Entrez Gene: 797853 Zebrafish Omim: 312180 Human SwissProt: P49459 Human SwissProt: P63146 Human SwissProt: Q9Z255 Mouse Unigene: 379466 Human Unigene: 395649 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
泛素结合酶E2蛋白A抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 **学 表观遗传学
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid