有丝分裂阻滞缺陷2样蛋白2抗体
规格：1mg/1ml
英文名： Mad2L2
别名： Homolog of REV7 S cerevisiae; HREV7; MAD2 homolog; MAD2 like 2; MAD2 mitotic arrest deficient like 2; MAD2B; Mitotic Arrest Deficient 2 L2; Mitotic arrest deficient homolog like 2; Mitotic arrest defi
分子量： 23kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human Mad2L2
交叉反应：Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit,
细胞定位：细胞核 细胞浆
有丝分裂阻滞缺陷2样蛋白2抗体产品介绍：background: MAD2L2 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L2 is a homolog of MAD2L1. Function: Adapter protein able to interact with different proteins and involved in different biological processes. Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the second polymerase switching in translesion DNA synthesis. Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions. May also regulate another aspect of cellular response to DNA damage through regulation of the JNK-mediated phosphorylation and activation of the transcriptional activator ELK1. Inhibits the FZR1- and probably CDC20-mediated activation of the anaphase promoting complex APC thereby regulating progression through the cell cycle. Regulates TCF7L2-mediated gene transcription and may play a role in epithelial-mesenchymal transdifferentiation. Subunit: Homooligomer (Probable). Interacts with REV1. Interacts with ADAM9. Interacts with CHAMP1. Interacts with REV3L有丝分裂阻滞缺陷2样蛋白2抗体. Interacts with FZR1 (in complex with the anaphase promoting complex APC). Interacts with CDC20; PubMed:11459825 could not detect the interaction. Interacts with RAN. Interacts with ELK1; the interaction is direct and recruits MAD2L2 to ELK1-specific promoters. May interact with the JNK kinases MAPK8 and/or MAPK9 to stimulate ELK1 phosphorylation and transcriptional activity upon DNA damage. Interacts with TCF7L2; prevents its binding to promoters and negatively modulates its transcriptional activity. Interacts with YY1AP1. Interacts with S. flexneri protein ipaB; prevents the interaction of MAD2L2 with FZR1 and CDC20 resulting in an activation of the anaphase-promoting complex APC and a cell cycle arrest. Interacts with PRCC; the interaction is direct. Interacts with POGZ. Subcellular Location: Nucleus. Cytoplasm, cytoskeleton, spindle. Cytoplasm.有丝分裂阻滞缺陷2样蛋白2抗体 Tissue Specificity: Ubiquitously expressed. Similarity: Contains 1 HORMA domain. Database links: UniProtKB/Swiss-Prot: Q9UI95.2 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
有丝分裂阻滞缺陷2样蛋白2抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 细胞周期蛋白 细胞分化
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit