高尔基体外周膜蛋白p65抗体
规格：1mg/1ml
英文名： GRASP65
别名： Golgi peripheral membrane protein p65; Golgi phosphoprotein 5; Golgi reassembly and stacking protein 1; Golgi reassembly and stacking protein 65 kDa; Golgi reassembly stacking protein 1 65kDa; Golgi r
分子量： 46kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human GRASP65
交叉反应：Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Xenopuslaevis
细胞定位：细胞浆 细胞膜
产高尔基体外周膜蛋白p65抗体品介绍：background: Stacking factor involved in the postmitotic assembly of Golgi stacks from mitotic Golgi fragments. Key structural protein required for the maintenance of the Golgi apparatus integrity: its caspase-mediated cleavage is required for fragmentation of the Golgi during apoptosis. Also mediates, via its interaction with GM130, the docking of transport vesicles with the Golgi membranes. Subunit: Homodimer. Forms higher order oligomers under interphase but not mitotic conditions. Dimers of the protein on one membrane might be able to interact with dimers on another and so stack cisternae. Interacts with the C-terminus of GM130 under both mitotic and non-mitotic conditions. The interaction is critical for the correct targeting of both proteins to the cis-Golgi. The complex binds to the vesicle 高尔基体外周膜蛋白p65抗体docking protein p115. Interacts with TMED2 and TMED3 (By similarity). Subcellular Location: Golgi apparatus, cis-Golgi network membrane; Post-translational modifications: Phosphorylated by CDC2/B1 and PLK kinases during mitosis. Phosphorylation cycle correlates with the cisternal stacking cycle. Phosphorylation of the homodimer prevents the association of dimmers into higher order oligomers, leading to cisternal 高尔基体外周膜蛋白p65抗体unstacking. Target for caspase-3 cleavage during apoptosis. The cleavage contributes to Golgi fragmentation and occurs very early in the execution phase of apoptosis (By similarity). Similarity: Belongs to the GORASP family. Contains 1 PDZ (DHR) domain. Database links: UniProtKB/Swiss-Prot: Q9BQQ3.3 Entrez Gene: 64689 Human Omim: 606867 Human SwissProt: Q9BQQ3 Human Unigene: 613614 Human Unigene: 721946 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
高尔基体外周膜蛋白p65抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 **学 细胞凋亡 细胞周期蛋白 细胞自噬
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid