中心体蛋白55kDa抗体
规格：1mg/1ml
英文名： CEP55
别名： C10orf3; Centrosomal protein 55kDa; Centrosomal protein of 55 kDa; CEP 55; FLJ10540; Up regulated in colon cancer 6; URCC 6; URCC6; CEP55_HUMAN.
分子量： 54kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human CEP55
交叉反应：Human, Mouse, Rat, Dog, Pig, Horse, Sheep,
细胞定位：细胞浆
中心体蛋白55kDa抗体产品介绍：background: CEP55 (Centrosomal protein 55kDa) is essential for mitotic exit and cytokinesis. It is not required for microtubule nucleation. Function: Plays a role in mitotic exit and cytokinesis. Not required for microtubule nucleation. Recruits PDCD6IP and TSG101 to midbody during cytokinesis. Subunit: Homodimer. Interacts (phosphorylated on Ser-425 and Ser-428) with PLK1. Interacts with AKAP9; the interaction occurs in interphase and is lost upon mitotic entry. Interacts with PCNT; the interaction occurs in interphase and is lost upon mitotic entry. Interacts with PDCD6IP; the interaction is direct; CEP55 binds PDCD6IP in a 2:1 stoechiometry; PDCD6IP competes with TSG101 for the same binding site. Interacts with TSG101; TSG101 competes with PDCD6IP for the same binding site; interaction is required for cytokinesis but not for viral budding. Interacts with MVB12A, VPS37B, VPS37C and VPS28. Subcellular Location: Cytoplasm, cytoskeleton, centrosome, centriole. Cytoplasm, cytoskeleton, centrosome. Cleavage furrow. Midbody. Note=Present at the centrosomes at interphase. A small portion is associated preferentially with the mother centriole, whereas the majority localizes to the pericentriolar material. During mitosis, loss of affinity for the centrosome at the onset of prophase and diffusion throughout the cell. This dissociation from the centrosome is phosphorylation-dependent. May remain中心体蛋白55kDa抗体 localized at the centrosome during mitosis in certain cell types. Appears at the cleavage furrow in late anaphase and in the midbody in cytokinesis. Tissue Specificity: Widely expressed, mostly in proliferative tissues. Highly expressed in testis. Intermediate levels in ***** and fetal thymus, as well as in various cancer cell lines. Low levels in different parts of the digestive tract, bone marrow, lymph nodes, placenta, fetal heart and fetal spleen. Hardly detected in brain. Post-translational modifications: There is a hierachy of phosphorylation, where both Ser-425 and 中心体蛋白55kDa抗体Ser-428 are phosphorylated at the onset of mitosis, prior to Ser-436. Phosphorylation at Ser-425 and Ser-428 is required for dissociation from the centrosome at the G2/M boundary. Phosphorylation at the 3 sites, Ser-425, Ser-428 and Ser-436, is required for protein function at the final stages of cell division to complete cytokinesis successfully. Database links: UniProtKB/Swiss-Prot: Q53EZ4.3 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
中心体蛋白55kDa抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 细胞周期蛋白 细胞分化
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid
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