肝癌相关抗原57抗体
规格：1mg/1ml
英文名： HCA57
别名： DnaJ (Hsp40) homolog subfamily A member 3; DnaJ homolog subfamily A member 3 mitochondrial precursor; DnaJ protein Tid 1; DnaJ protein Tid1; HCA57; Hepatocellular carcinoma associated antigen 57; High
分子量： 52kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human TID1/HCA
交叉反应：Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep,
细胞定位：细胞浆 细胞膜
肝癌相关抗原57抗体产品介绍：background: TID1 is a human homolog of the Drosophila tumor suppressor lethal tumerous imaginal discs and encodes two mitochondrial matrix localized splice variants of human Tid1 designated hTid1S and hTid1L. These proteins are the conserved members of the DnaJ family of proteins which act as cochaperons for mitochondrial Hsp70. They contain a conserved tetrahedrical J domain which binds to Hsp70 chaperones and activates their ATPase activity. Expression of hTid1L increases apoptosis induced by DNA damaging agents as mitomycin C and TNF alpha. A J domain mutant of hTid1L can dominantly suppress apoptosis and in sharp contrast the J domain mutant of hTid1S increases apoptosis. Expression of hTid1S and hTid1L affects cytochrome c release from the mitochondria and caspase 3 activation, while activation of caspase 8 is unaffected. It is strongly suggested that these two splice variants exert their anti and pro apoptotic effects through discrete substrates and activities. Hence the relative abundance of these proteins or their substrates may allow the mitochondria to dampen or enhance the apoptotic signals. Function: Modulates apoptotic signal transduction or effector structures within the mitochondrial matrix. Affect cytochrome C release from the mitochondria and caspase 3 activation, but not caspase 8 activation. Isoform 1 increases apoptosis triggered by both TNF and the 肝癌相关抗原57抗体DNA-damaging agent mytomycin C; in sharp contrast, isoform 2 suppresses apoptosis. Can modulate IFN-gamma-mediated transcriptional activity. Isoform 2 may play a role in neuromuscular junction development as an effector of the MUSK signaling pathway. Subunit: Interacts with JAK2, HSPA9B and IFN-gammaR2 chain. Interacts with Ras GTPase-activating protein 1 (RASA1). Isoform 2 interacts with MUSK (via the cytoplasmic domain) (By similarity). Subcellular Location: Mitochondrion matrix. Cytoplasm, cytosol (By similarity). Cell junction, synapse, postsynaptic cell membrane; Peripheral membrane protein (By similarity). Note=Recruited to the postsynaptic cell membrane of the neuromuscular junction through interaction with MUSK (By similarity). Tissue Specificity: Widely expressed with highest levels in heart, liver, lung and skeletal muscles. Also expressed 肝癌相关抗原57抗体in keratinocytes. Post-translational modifications: Tyrosine phosphorylated (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Contains 1 CR-type zinc finger. Contains 1 J domain. Gene ID: 9093 Database links: Entrez Gene: 9093 Human Entrez Gene: 83945 Mouse Entrez Gene: 360481 Rat Omim: 608382 Human SwissProt: Q96EY1 Human SwissProt: Q99M87 Mouse Unigene: 459779 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
肝癌相关抗原57抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 信号转导 细胞凋亡
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid