蛋白激酶A催化亚基抗体
规格：1mg/1ml
英文名： PRKACA
别名： cAMP Protein Kinase Catalytic subunit; cAMP dependent protein kinase alpha catalytic subunit; cAMP dependent protein kinase beta catalytic subunit; cAMP dependent protein kinase catalytic beta subunit
分子量： 40kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human cAMP Pro
交叉反应：Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep,
蛋白激酶A催化亚基抗体细胞定位：细胞核 细胞浆
产品介绍：background: This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms. [provided by RefSeq, Jan 2015] Function: CREB, SMAD6 and PKD1 and has multiple functions in cellular differentiation and epithelial morphogenesis. Regulates myeloid cell differentiation through SMAD6 phosphorylation. Involved in nephrogenesis by stimulating renal epithelial cell migration and tubulogenesis. Also involved in angiogenesis through stimulation of endothelial cell proliferation, migration and vascular-like structure formation Subunit: Like other cAMP-dependent protein kinases, the inactive holoenzyme is probably composed of 2 PRKX catalytic subunits and a dimer of regulatory subunits. Interacts (cAMP-dependent) specifically with the regulatory subunits PRKAR1A and PRKAR1B. Compared to other cAMP-dependent serine/threonine protein kinases, does not interact with the 2 other PKA regulatory subunits PRKAR2A and PRKAR2B. Interacts with cAMP-dependent protein kinase inhibitor/PKI proteins; 蛋白激酶A催化亚基抗体inhibits PRKX. Interacts with GPKOW. Interacts with SMAD6. Interacts with PKD1; involved in differentiation and controlled morphogenesis of the kidney. Interacts with PIN1 (via WW domain). Subcellular Location: Cytoplasmic and Nuclear Tissue Specificity: Widely expressed (at protein level). Specifically expressed in blood by macrophages and granulocytes according to PubMed:9860982. Post-translational modifications: Phosphorylated; autophosphorylates in vitro. DISEASE: A chromosomal aberration involving PRKX is a cause of sex reversal disorder. Translocation t(X;Y)(p22;p11) with PRKY. Chromosomal translocations proximal to PRKY account for about 30% of the cases of sex reversal disorder in XX males and XY females. Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily. Gene ID: 5566 Database links: Entrez Gene: 282322 Cow Entrez Gene: 282323 Cow Entrez Gene: 403556 Dog Entrez Gene: 5566 Human Entrez Gene: 5567 Human Entrez蛋白激酶A催化亚基抗体 Gene: 18747 Mouse Entrez Gene: 18749 Mouse Entrez Gene: 397494 Pig Entrez Gene: 397652 Pig Entrez Gene: 25636 Rat Entrez Gene: 293508 Rat Entrez Gene: 446502 Xenopus laevis Omim: 176892 Human Omim: 601639 Human SwissProt: P00517 Cow SwissProt: P05131 Cow SwissProt: Q8MJ44 Dog SwissProt: P17612 Human SwissProt: P22694 Human SwissProt: P05132 Mouse SwissProt: P68181 Mouse SwissProt: P05383 Pig SwissProt: P36887 Pig SwissProt: P27791 Rat SwissProt: P68182 Rat Unigene: 487325 Human Unigene: 631630 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
蛋白激酶A催化亚基抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：肿瘤 细胞生物 信号转导 激酶和磷酸酶
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid