NCK衔接蛋白1/2抗体
规格：1mg/1ml
英文名： NCK adaptor protein 1/2
别名： Nck 1/2; NCK adaptor protein 1 + 2 GRB4; melanoma NCK protein; MGC12668; NCK; NCK adaptor protein 1; NCK adaptor protein 2; NCK tyrosine kinase; NCKalpha; NCKbeta; NCK1_HUMAN；NCK12_HUMAN； non catalyti
分子量： 43kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human NCK adap
交叉反应：Human, Mouse, Rat,
细胞定位：细胞核 细胞浆
NCK衔接蛋白1/2抗体产品介绍：background: The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Jun 2010] Function: NCK1 is one of the adaptor proteins which mediate specific protein-protein interactions in signaling processes. Adaptor proteins usually contain several domains like SH2 and SH3 which allow specific interactions with other specific proteins. NCK1 and NCK2 show high sequence identity (68%) and have three SH3 domains and a C-terminal SH2 domain. Both of them bind receptor tyrosine kinases such as PDGFR and other tyrosine phosphorylated proteins via their SH2 domains. Various molecules which interact with SH domains of Nck and regulate signaling process of actin cytoskeleton reorganization have been identified. Ncks are thought to have important functions in the development of mesodermal structures during embryogenesis, linked to a role in cell movement and cytoskeletal reorganization. Ncks also have a function in modulating mRNA translation at the level of initiation by interacting eukayotic initiation factor 2 (eIF2). Under stressed conditions, protein synthesis is reduced by inhibiting the activity of eIF2 through phosphorylation, transiently inhibiting recycling of eIF2 into its active form. Subcellular Location: NCK衔接蛋白1/2抗体Cytoplasm. Endoplasmic reticulum. Nucleus. Note=Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Import into the nucleus requires the interaction with SOCS7. Predominantly nuclear following genotoxic stresses, such as UV irradiation, hydroxyurea or mitomycin C treatments. Post-translational modifications: Phosphorylated on Ser and Tyr residues. Phosphorylated in response to activation of EGFR and FcERI. Phosphorylated by activated PDGFRB. Similarity: Contains 1 SH2 domain. Contains 3 SH3 domains. Gene ID: 4690；8440 Database links:NCK衔接蛋白1/2抗体 Entrez Gene: 4690 Human Entrez Gene: 8440 Human Entrez Gene: 17973 Mouse Entrez Gene: 17974 Mouse Entrez Gene: 300955 Rat Entrez Gene: 316369 Rat Omim: 600508 Human Omim: 604930 Human SwissProt: O43639 Human SwissProt: P16333 Human SwissProt: Q8BQ28 Mouse SwissProt: Q99M51 Mouse SwissProt: B2RZ33 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
NCK衔接蛋白1/2抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：肿瘤 细胞生物 信号转导 激酶和磷酸酶
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid