多核苷酸磷酸化酶蛋白抗体
规格：1mg/1ml
英文名： PNPT1
别名： OLD35; PNPASE; PNPase 1; Polynucleotide phosphorylase like protein; Polyribonucleotide nucleotidyltransferase 1, mitochondrial; PNPT1_HUMAN.
分子量： 81kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human PNPT1
交叉反应：Human, Mouse, Rat, Dog, Cow, Horse, Sheep,
细胞定位：细胞浆 细胞膜
多核苷酸磷酸化酶蛋白抗体产品介绍：background: PNPT1 is a subunit of the exosome complex, which is involved in 3-prime-to-5-prime exoribonuclease activity for RNA processing and degradation. It contains one KH domain and one S1 motif domain. Function: RNA-binding protein implicated in numerous RNA metabolic processes. Hydrolyzes single-stranded polyribonucleotides processively in the 3'-to-5' direction. Mitochondrial intermembrane factor with RNA-processing exoribonulease activity. Component of the mitochondrial degradosome (mtEXO) complex, that degrades 3' overhang double-stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner. Required for correct processing and polyadenylation of mitochondrial mRNAs. Plays a role as a cytoplasmic RNA import factor that mediates the translocation of small RNA components, like the 5S RNA, the RNA subunit of ribonuclease P and the mitochondrial RNA-processing (MRP) RNA, into the mitochondrial matrix. Plays a role in mitochondrial morphogenesis and respiration; regulates the expression of the electron transport chain (ETC) components at the mRNA and protein levels.多核苷酸磷酸化酶蛋白抗体 In the cytoplasm, shows a 3'-to-5' exoribonuclease mediating mRNA degradation activity; degrades c-myc mRNA upon treatment with IFNB1/IFN-beta, resulting in a growth arrest in melanoma cells. Regulates the stability of specific mature miRNAs in melanoma cells; specifically and selectively degrades miR-221, preferentially. Plays also a role in RNA cell surveillance by cleaning up oxidized RNAs. Binds to the RNA subunit of ribonuclease P, MRP RNA and miR-221 microRNA. Subunit: Homotrimer; in free form. Homooligomer. Component of the mitochondrial degradosome (mtEXO) complex which is a heteropentamer containing 2 copies of SUPV3L1 and 3 copies of PNPT1. Interacts with TCL1A; the interaction has no 多核苷酸磷酸化酶蛋白抗体effect on PNPT1 exonuclease activity. Subcellular Location: Cytoplasm. Mitochondrion. Mitochondrion intermembrane space; Peripheral membrane protein. Similarity: Belongs to the polyribonucleotide nucleotidyltransferase family. Contains 1 KH domain. Contains 1 S1 motif domain. Database links: UniProtKB/Swiss-Prot: Q8TCS8.2 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
多核苷酸磷酸化酶蛋白抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 细胞凋亡 表观遗传学
储存条件：
来源： Rabbit
外观： Lyophilized or Liquid