Nestin抗体经ICC, IHC, WB, FACS严格验证,品质保证.广泛种属反应性附有用户反馈信息.确保特异性!**的技术支持帮您更快得到实验结果,现货.我司提供8万种科研用专业抗体,经严格质控,特异性高,可重复性强,验证数..可与小鼠, 大鼠, 人样本反应并经WB, IHC, ICC实验严格验证。所有产品均提供质保服务,中国75%以上现货。
产品编号bs-8203R
英文名称Nestin
中文名称巢蛋白/神经上皮干细胞蛋白抗体
别 名ESTM 46; FLJ 21841; FLJ21841; Intermediate filament protein; Nbla00170; Nes; NEST_RAT; Nestin.
规格100ul 200ul
研究领域细胞生物 发育生物学 神经生物学 干细胞 细胞类型标志物
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Cow,
Nestin抗体产品应用ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量177kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Nestin
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
Nestin抗体产品介绍background:
Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.
Function:
Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Subunit:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3.
Subcellular Location:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, tis protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3
Tissue Specificity:
CNS stem cells.
Post-translational modifications:
Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
Similarity:
Belongs to the intermediate filament family.
SWISS:
P48681
Gene ID:
10763
Database links:
Nestin抗体溶解方法:
1、在我们公司购买抗体后,查看标签上的规格,如:0.1ml、0.2ml
2、 以规格为0.1ml的冻干粉抗体为例(部分抗体为液体,无需再溶解):
溶解方法一:用移液器加0.1ml双蒸水到试管内,混匀,-20℃保存;
若为其它规格(如:0.2ml)的冻干粉抗体,则加与标签上规格一致的(如:0.2ml)双蒸水溶解,混匀,-20℃保存。
溶解方法二:用移液器加 0.05ml(规格的 50%)双蒸水到试管内,再加 0.05ml(规格的 50%)
的甘油到试管内,混匀,-20℃保存; 若为其它规格(如:0.2ml)的冻干粉抗体,则加标签上规格的 50%(如:0.1ml)双蒸水,再加标签上规格的50%(如:0.1ml)甘油溶解,混匀,-20℃保存。
3、抗体的分装
抗体溶解后,将其分装成 5-10支(以0.1mlNestin抗体为例,20ulx5支或 10ulx10支),-20℃保存,用一支取一支,避免反复冻溶。
另:预试验可做几个梯度1:100、1:200、1:400稀释,如果背景高,还可再稀释,选一个*佳的稀释比例,再正式做,效果*好。工作液可用“抗体稀释液”稀释。
后续试验时,再使用对应的缓冲液稀释成工作液,即用即配。
我们的抗体(冻干粉),在常温放置下,至少一个月有效;若在-20℃下保存(推荐),至少三年有效。
Nestin抗体关联产品列表:
bs-0006R YES 合格 Nestin 巢蛋白/神经上皮干细胞蛋白抗体
bs-0008R YES 合格 合格 Nestin 巢蛋白/神经上皮干细胞蛋白抗体
bs-0009R No 合格 alpha-Synuclein 核突触蛋白-α抗体(N端)
bs-0013R YES 合格 合格 Cytochrome C 细胞色素C抗体
bs-0014R YES 合格 IGF 1 胰岛素样生长因子1抗体
bs-0016R YES 合格 合格 Tyrosine Hydroxylase 酪氨酸羟化酶抗体
bs-0017R No 合格 Galanin 甘丙肽/神经节肽抗体
bs-0018R No 合格 IDE 胰岛素降解酶抗体
bs-0019R YES 合格 合格 osteopontin 骨桥蛋白/分泌型磷蛋白1抗体
bs-0022R YES 合格 合格 ERK1 + ERK2 丝裂原活化蛋白激酶1/ERK 1/2抗体
bs-0023M No 合格 GSK-3 beta 糖原合酶激酶-3β抗体
bs-0024R No 合格 合格 presenilin 1 早老素蛋白-1抗体
bs-0026R YES 合格 osteopontin 骨桥蛋白/分泌型磷蛋白1抗体
bs-0027R YES 合格 Melatonin Receptor 1A 褪黑素受体1A/松果体素受体1A抗体
bs-0028R YES 合格 GSK-3 Beta 糖原合酶激酶-3β抗体
bs-0029R YES 合格 PP2A alpha + beta 蛋白质磷酸酶-2A抗体
bs-0031R No 合格 合格 BTG2 B细胞迁移基因2抗体
bs-0032R YES 合格 合格 合格 Bcl-2 Bcl-2抗体
bs-0033R YES 合格 P53 protein(wt-p53) 肿瘤抑制基因P53蛋白/野生型P53抗体
bs-0035R No 合格 CREB-1 环腺苷酸应答元件结合蛋白抗体
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