beta-Actin抗体经ICC, IHC, WB, FACS严格验证,品质保证.广泛种属反应性附有用户反馈信息.确保特异性!**的技术支持帮您更快得到实验结果，现货.我司提供8万种科研用专业抗体,经严格质控,特异性高,可重复性强,验证数..可与小鼠, 大鼠, 人样本反应并经WB, IHC, ICC实验严格验证。所有产品均提供质保服务,中国75%以上现货。
产品编号bs-0061R
英文名称beta-Actin (Loading Control)
中文名称β-肌动蛋白/β-Actin 抗体（内参抗体）
别 名Beta Actin; beta-Actin; ACTB; Actin cytoplasmic 1; Actin, beta; Beta actin; beta cytoskeletal actin;A X actin like protein; ACTB; Actin cytoplasmic 1; alpha sarcomeric Actin; Actx; Beta cytoskeletal actin; Melanoma X actin; PS1TP5BP1; ACTB_HUMAN.
JournalPMIDIFApplicationBiomaterials (2013)238100817.6040WBBiomaterials (2013)237779167.6040WBNanotoxicology (2015)263995856.4110WBArteriosclerosis, thrombosis, and vascular biology (2008)185997966.3380WBArchives of toxicology (2012)224223405.2150WBMolecular Neurobiology (2015)263855765.1370WBMolecular Cancer (2013)242156325.1300WBBritish Journal of Pharmacology (2014)248353595.0670 WB Endocrinology (2015) 26287402 4.5030 WB Molecular Pharmacology (2013)234702874.4110WBCancer letters (2007)182268554.2580WBBiochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids (2010)203822594.1340WBNanotechnology (2013)234259413.8420WB Nanotechnology (2013) 23425941 3.8420 WB Proteomics (2016) 27173832 3.8100 WB The Journal of Nutritional Biochemistry (2014) 24794015 3.7940 WB PloS One (2012) 23028660 3.7300 WB PloS One (2013) 23922700 3.7300 WB PloS One (2013) 24098763 3.7300 WB Cancer Immunology, Immunotherapy (2013) 24077852 3.6370 WB Colloids and Surfaces B: Biointerfaces (2012) 22503631 3.5540 WB PLoS One (2015) 26266800 3.5340 WB Cellular Physiology and Biochemistry (2014) 24556756 3.4150 WB Free radical research (2007) 17907002 3.2790 WB Developmental & Comparative Immunology (2012) 23178402 3.2380 WB/IP Developmental & Comparative Immunology (2013) 23545285 3.2380 WB Developmental & Comparative Immunology (2013) 23500512 3.2380 WB Developmental & Comparative Immunology (2013) 23932982 3.2380 WB Developmental & Comparative Immunology (2012) 23178402 3.2380 WB International Journal of Biological Sciences (2014) 24719562 3.1680 WB Toxicology Letters (2011) 21232584 3.1450 WB Neuroscience (2011) 21219973 3.1220 WB Diabetes/Metabolism Research and Reviews (2012) 21928330 2.9680 WB Chemico-Biological Interactions (2012) 24025711 2.9670 WB Fish & Shellfish Immunology (2012) 23063540 2.9640 WB Journal of Cancer Research and Clinical Oncology (2012) 22806311 2.9140 WB Food and Chemical Toxicology (2016) 27237588 2.9000 WB Brain Research Bulletin (2016) 26876758 2.7180 WB Life Sciences (2016) 26924495 2.7020 WB Nutrition and Cancer (2014) 24660968 2.6950 WB Neurochemistry international (2013) 24220540 2.6590 WB Neurochemistry international (2013) 24274996 2.6590 WB Neurochemistry international (2011) 21185898 2.6590 WB Life Science Journal (2013) Others 2.5550 WB Toxicology and Applied Pharmacology (2016) 27105818 2.5320 WB Tumor Biology (2013) 23539435 2.5180 WB Acta Pharmacologica Sinica (2015) 25937633 2.4960 WB World Journal of Gastroenterology (2015) 25663771 2.4330 WB Biochemical and Biophysical Research Communications (2013) 24211202 2.4060 WB Acta Pharmacologica Sinica (2014) 24632846 2.3540 WB Acta Pharmacologica Sinica (2014) 24909515 2.3540 WB Molecular and Cellular Biochemistry (2011) 21553022 2.3290 WB Molecular and Cellular Biochemistry (2014) 24833465 2.3290 WB Rheumatology international (2010) 19847436 2.2140 WB Medical Oncology (2013) 23536000 2.1470 WB Reproductive Biology and Endocrinology (2013) 23452984 2.1400 WB Neurochemical Research (2013) 23780673 2.1250 WB Virology Journal (2011) 21392403 2.0900 WB Respiratory physiology & neurobiology (2013) 23665052 2.0510 WB International Journal of Clinical and Experimental Pathology (2015) 26261494 1.8910 WB Research in Veterinary Science (2012) 23164636 1.7740 WB Cellular Immunology (2013) 23774531 1.7430 WB Applied Biochemistry and Biotechnology (2016) 27193254 1.7400 WB Poultry Science (2014) 24570433 1.7280 WB Nephrology (2011) 21771176 1.6880 WB Biological Trace Element Research (2015) 25876085 1.6080 WB Biological trace element research (2013) 23575899 1.3070 WB Others 1.2770 WB Brazilian Journal of Medical and Biological Research (2014) 25517918 1.1390 WB Experimental & Clinical Cardiology (2013) 23940455 1.1000 WB World Journal of Surgical Oncology (2014) 24885858 1.0900 WB CHINESE MEDICAL JOURNAL-BEIJING-ENGLISH EDITION (2009) 19236815 0.9010 WB Pediatrics International (2016) 27097979 0.7300 WB Experimental and Therapeutic Medicine (2014) 24396436 0.3440 WB
规格价格50ul/580元 购买 100ul/920元 购买 200ul/1600元 购买 大包装/询价
说 明 书50ul 100ul 200ul
产品类型内参抗体
研究领域肿瘤 细胞生物 信号转导 细胞骨架
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit, Sheep, Bee, Fish, Guinea Pig, Hamster, Goat, Cat, mt,op
beta-Actin抗体产品应用WB=1:5000-20000 ELISA=1:5000-20000 IHC-P=1:500-1000 IHC-F=1:500-1000 Flow-Cyt=1μg/Test IF=1:100-1000 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量177kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Nestin
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
beta-Actin抗体产品介绍background:
Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.
Function:
Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Subunit:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3.
Subcellular Location:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, tis protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3
Tissue Specificity:
CNS stem cells.
Post-translational modifications:
Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
Similarity:
Belongs to the intermediate filament family.
SWISS:
P48681
Gene ID:
10763
Database links:
beta-Actin抗体溶解方法：
1、在我们公司购买抗体后，查看标签上的规格，如：0.1ml、0.2ml
2、 以规格为0.1ml的冻干粉抗体为例（部分抗体为液体，无需再溶解）：
溶解方法一：用移液器加0.1ml双蒸水到试管内，混匀，-20℃保存；
若为其它规格（如：0.2ml）的冻干粉抗体，则加与标签上规格一致的（如：0.2ml）双蒸水溶解，混匀，-20℃保存。
溶解方法二：用移液器加 0.05ml（规格的 50%）双蒸水到试管内，再加 0.05ml（规格的 50%）
的甘油到试管内，混匀，-20℃保存； 若为其它规格（如：0.2ml）的冻干粉抗体，则加标签上规格的 50%（如：0.1ml）双蒸水，再加标签上规格的50%（如：0.1ml）甘油溶解，混匀，-20℃保存。
3、抗体的分装
抗体溶解后，将其分装成 5-10支（以0.1mlbeta-Actin抗体为例，20ulx5支或 10ulx10支），-20℃保存，用一支取一支，避免反复冻溶。
另：预试验可做几个梯度1：100、1：200、1：400稀释，如果背景高，还可再稀释，选一个*佳的稀释比例，再正式做，效果*好。工作液可用“抗体稀释液”稀释。
后续试验时，再使用对应的缓冲液稀释成工作液，即用即配。
我们的抗体（冻干粉），在常温放置下，至少一个月有效；若在-20℃下保存（推荐），至少三年有效。
beta-Actin抗体关联产品列表：
bs-0952R No 合格 LH 促黄体生成素抗体
bs-0954R YES 合格 ET-1 内皮素-1抗体
bs-0961R No 合格 合格 Leptin receptor 瘦素受体抗体
bs-0962R No 合格 SLC34A2 磷酸钠协同转运蛋白抗体
bs-0975R YES 合格 SP1 转录生长因子SP1抗体
bs-0979R No 合格 CD38 CD38抗体
bs-0980R No 合格 CD38 CD38抗体
bs-0982R YES 合格 合格 phospho-NFKB p65(Ser536) 磷酸化细胞核因子抗体
bs-0988R No 合格 合格 CD71/Transferrin receptor 转铁蛋白受体抗体
bs-0990R YES 合格 HPV16 E6 protein 人类**状瘤病毒16抗体
bs-0991R No 合格 HPV18 E6 protein 人类**状瘤病毒18 E6抗体
bs-10005R YES 合格 c-Kit 干细胞生长因子受体/细胞表面分化抗原抗体
bs-10006R YES 合格 合格 CTLA4 细胞毒性T细胞抗原-4抗体
bs-10007R No 合格 EGFR 表皮生长因子受体抗体
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