Substance P抗体经ICC, IHC, WB, FACS严格验证,品质保证.广泛种属反应性附有用户反馈信息.确保特异性!**的技术支持帮您更快得到实验结果,现货.我司提供8万种科研用专业抗体,经严格质控,特异性高,可重复性强,验证数..可与小鼠, 大鼠, 人样本反应并经WB, IHC, ICC实验严格验证。所有产品均提供质保服务,中国75%以上现货。
产品编号bs-0065R
英文名称Substance P
中文名称P物质抗体
别 名Hs.2563; Neurokinin 1; Neurokinin 2; Neurokinin A; Neurokinin alpha; Neuromedin L; Neuropeptide gamma; Neuropeptide K; NK2; NKA; NKNA; PPT; Protachykinin 1 precursor; Substance K; Substance P; TAC1; TAC2; Tachykinin 1; Tachykinin 2; Tachykinin precursor 1; Tachykinin1; TACI; TKN1_HUMAN.
JournalPMIDIFApplicationNeurobiology of Aging (2015)256196624.8530IHC-FBrain research bulletin (2013)236885192.9350IHCPurinergic Signalling (2013)237541202.6350IHCJournal of Huazhong University of Science and Technology, Medical Science (2014)251357350.7790WB
规格价格50ul/780元 购买 100ul/1280元 购买 200ul/1980元 购买 大包装/询价
说 明 书50ul 100ul 200ul
研究领域神经生物学 新陈代谢
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Pig, Cow, Horse, Rabbit, Sheep, Guinea Pig, G
Substance P抗体产品应用ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量177kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Nestin
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
Substance P抗体产品介绍background:
Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.
Function:
Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Subunit:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3.
Subcellular Location:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, tis protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3
Tissue Specificity:
CNS stem cells.
Post-translational modifications:
Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
Similarity:
Belongs to the intermediate filament family.
SWISS:
P48681
Gene ID:
10763
Database links:
Substance P抗体溶解方法:
1、在我们公司购买抗体后,查看标签上的规格,如:0.1ml、0.2ml
2、 以规格为0.1ml的冻干粉抗体为例(部分抗体为液体,无需再溶解):
溶解方法一:用移液器加0.1ml双蒸水到试管内,混匀,-20℃保存;
若为其它规格(如:0.2ml)的冻干粉抗体,则加与标签上规格一致的(如:0.2ml)双蒸水溶解,混匀,-20℃保存。
溶解方法二:用移液器加 0.05ml(规格的 50%)双蒸水到试管内,再加 0.05ml(规格的 50%)
的甘油到试管内,混匀,-20℃保存; 若为其它规格(如:0.2ml)的冻干粉抗体,则加标签上规格的 50%(如:0.1ml)双蒸水,再加标签上规格的50%(如:0.1ml)甘油溶解,混匀,-20℃保存。
3、抗体的分装
抗体溶解后,将其分装成 5-10支(以0.1mlSubstance P抗体为例,20ulx5支或 10ulx10支),-20℃保存,用一支取一支,避免反复冻溶。
另:预试验可做几个梯度1:100、1:200、1:400稀释,如果背景高,还可再稀释,选一个*佳的稀释比例,再正式做,效果*好。工作液可用“抗体稀释液”稀释。
后续试验时,再使用对应的缓冲液稀释成工作液,即用即配。
我们的抗体(冻干粉),在常温放置下,至少一个月有效;若在-20℃下保存(推荐),至少三年有效。
Substance P抗体关联产品列表:
bs-1000R YES 合格 合格 CNPase 2’,3’-环腺苷酸-3’-磷酸二酯酶抗体
bs-10011R No 合格 Occludin 紧密连接蛋白抗体
bs-10014R No 合格 AVPR2 精氨酸加压素受体2抗体
bs-10020R No 合格 合格 HADHSC 短链L-3羟烷基辅酶A脱氢酶抗体
bs-10021R YES 合格 合格 NALP3/CIAS1 细胞凋亡诱导蛋白NALP3抗体
bs-10024R No 合格 HDAC3/HD3 组蛋白去乙酰化酶3抗体
bs-10027R YES 合格 NCR1 细胞毒性受体NK-p46抗体
bs-10029R No 合格 Nectin 4 脊髓灰质炎病毒受体相关蛋白4抗体
bs-10044R No 合格 Newcastle disease virus 鸡新城疫病毒抗体
bs-1004R YES 合格 ACE2 血管紧张素转换酶2抗体
bs-10053R No 合格 合格 DC-SIGN 细胞间粘附分子非整合素蛋白3抗体
bs-10054R No 合格 GPR48 G蛋白偶联受体48抗体
bs-10055R No 合格 RIP1 受体相互作用蛋白1抗体
bs-10058R No 合格 MMP19 基质金属蛋白酶18/19抗体
bs-10062R No 合格 合格 HGFA Inhibitor 2 肝细胞生长因子激活抑制蛋白2抗体
bs-10063R No 合格 Lymphotoxin Beta 肿瘤坏死因子C/****β抗体
- 温馨提示:为规避购买风险,建议您在购买前务必确认供应商资质与产品质量。
- 免责申明:以上内容为注册会员自行发布,若信息的真实性、合法性存在争议,平台将会监督协助处理,欢迎举报