CD31抗体

CD31抗体经ICC, IHC, WB, FACS严格验证,品质保证.广泛种属反应性附有用户反馈信息.确保特异性!**的技术支持帮您更快得到实验结果，现货.我司提供8万种科研用专业抗体,经严格质控,特异性高,可重复性强,验证数..可与小鼠, 大鼠, 人样本反应并经WB, IHC, ICC实验严格验证。所有产品均提供质保服务,中国75%以上现货。

产品编号bs-0195R

英文名称CD31

中文名称血小板内皮细胞黏附分子1抗体

别    名platelet endothelial cell adhesion molecule precursor-1; PECAM-1; PECAM1; Adhesion molecule; CD31 antigen; CD31 EndoCAM; Endocam; FLJ34100; FLJ58394; GPIIA; Pecam 1; PECA1_HUMAN; PECAM 1 CD31 EndoCAM; PECA1; Pecam1; Platelet endothelial cell adhesion molecule; Platelet/endothelial cell adhesion molecule 1; Adhesion molecule; Platelet/endothelial cell adhesion molecule.  

JournalPMIDIFApplicationBiomaterials (2013)232904687.6040IHC/IFBiomaterials (2013)238305807.6040IHCBiomaterials (2014)247681937.6040IHC-PBiochemical pharmacology (2007)178686504.5760IFPloS One (2012)231190013.7300IHC-PPLoS One (2014)253802453.5340FCMLiu, Yang, et al. (2014) Int J Biol Sci.10(4):448-56 (2014)247195623.1680IHC-PStem Cells International (2015)266490502.8130FCMMolecular biology reports (2012)222378622.5060ICCEuropean Surgical Research (2015)266849132.4740IHC-PEuropean Journal of Histochemistry (2015)26708182 2.0420 IHC-PGraefes Archive for Clinical and Experimental Ophthalmology (2014)245624651.9320IHC-PGraefes Archive for Clinical and Experimental Ophthalmology (2014)247894641.9320WB

规格价格50ul/780元 购买    100ul/1280元 购买    200ul/1980元 购买    大包装/询价

说 明 书50ul  100ul  200ul

研究领域肿瘤  心血管  细胞生物  **学  信号转导  干细胞  细胞粘附分子  细胞表面分子  糖蛋白  细胞类型标志物  血管内皮细胞  t-**细胞  内皮细胞  

抗体来源Rabbit

克隆类型Polyclonal

交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep,

CD31抗体产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 Flow-Cyt=1μg/Test （石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量177kDa

细胞定位细胞浆

性    状Lyophilized or Liquid

浓    度1mg/1ml

免 疫 原KLH conjugated synthetic peptide derived from human Nestin

亚    型IgG

纯化方法affinity purified by Protein A

储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMedPubMed

CD31抗体产品介绍background:

Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.

Function:

Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.

Subunit:

Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3.

Subcellular Location:

Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, tis protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3

Tissue Specificity:

CNS stem cells.

Post-translational modifications:

Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.

Similarity:

Belongs to the intermediate filament family.

SWISS:

P48681

Gene ID:

10763

Database links:

CD31抗体溶解方法：

1、在我们公司购买抗体后，查看标签上的规格，如：0.1ml、0.2ml

2、 以规格为0.1ml的冻干粉抗体为例（部分抗体为液体，无需再溶解）：

溶解方法一：用移液器加0.1ml双蒸水到试管内，混匀，-20℃保存；

若为其它规格（如：0.2ml）的冻干粉抗体，则加与标签上规格一致的（如：0.2ml）双蒸水溶解，混匀，-20℃保存。

溶解方法二：用移液器加 0.05ml（规格的 50%）双蒸水到试管内，再加 0.05ml（规格的 50%）

的甘油到试管内，混匀，-20℃保存； 若为其它规格（如：0.2ml）的冻干粉抗体，则加标签上规格的 50%（如：0.1ml）双蒸水，再加标签上规格的50%（如：0.1ml）甘油溶解，混匀，-20℃保存。

3、抗体的分装

抗体溶解后，将其分装成 5-10支（以0.1mlCD31抗体为例，20ulx5支或 10ulx10支），-20℃保存，用一支取一支，避免反复冻溶。

另：预试验可做几个梯度1：100、1：200、1：400稀释，如果背景高，还可再稀释，选一个*佳的稀释比例，再正式做，效果*好。工作液可用“抗体稀释液”稀释。

后续试验时，再使用对应的缓冲液稀释成工作液，即用即配。

我们的抗体（冻干粉），在常温放置下，至少一个月有效；若在-20℃下保存（推荐），至少三年有效。

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