Vimentin抗体

Vimentin抗体经ICC, IHC, WB, FACS严格验证,品质保证.广泛种属反应性附有用户反馈信息.确保特异性!**的技术支持帮您更快得到实验结果，现货.我司提供8万种科研用专业抗体,经严格质控,特异性高,可重复性强,验证数..可与小鼠, 大鼠, 人样本反应并经WB, IHC, ICC实验严格验证。所有产品均提供质保服务,中国75%以上现货。

产品编号bs-0756R

英文名称Vimentin

中文名称波形蛋白抗体

别    名FLJ36605; OTTHUMP; VIM; VIME_HUMAN; Vimentin.  

JournalPMIDIFApplicationAmerican Journal of Transplantation (2015)261043835.6830WBBiochemical Pharmacology (2013)235005465.0090ICCMolecular pharmacology (2014)254881834.1200WBPloS One (2013)240401633.7300WBInvestigative Ophthalmology & Visual Science (2014)244361923.4410IFEuropean Journal of Pharmaceutics and Biopharmaceutics (2015)260798313.3830IHC-FAmerican Journal of Reproductive Immunology (2012)227840283.3170ICCJournal of Cellular Biochemistry (2014)247006783.0620IHC-P Tumor Biology (2015) 25976503 2.8400WBBiochemical and Biophysical Research Communications (2013)236033612.4060ICCCell Biochemistry and Biophysics (2015)255676552.3800WBCell Biochemistry and Biophysics (2015)256567692.3800ICCJournal of Virological Methods (2014) 24642240 2.0650 IF International Journal of Clinical and Experimental Pathology (2015)266177871.8910WBJournal of International Medical Research (2013)240978300.9580IHC

规格价格50ul/780元 购买    100ul/1280元 购买    200ul/1980元 购买    大包装/询价

说 明 书50ul  100ul  200ul

研究领域肿瘤  细胞生物  **学  发育生物学  神经生物学  信号转导  干细胞  细胞骨架  肿瘤细胞生物标志物  

抗体来源Rabbit

克隆类型Polyclonal

交叉反应 Human, Mouse, Rat, Chicken, Pig, Cow,

Vimentin抗体产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量177kDa

细胞定位细胞浆

性    状Lyophilized or Liquid

浓    度1mg/1ml

免 疫 原KLH conjugated synthetic peptide derived from human Nestin

亚    型IgG

纯化方法affinity purified by Protein A

储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMedPubMed

Vimentin抗体产品介绍background:

Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.

Function:

Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.

Subunit:

Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3.

Subcellular Location:

Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, tis protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3

Tissue Specificity:

CNS stem cells.

Post-translational modifications:

Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.

Similarity:

Belongs to the intermediate filament family.

SWISS:

P48681

Gene ID:

10763

Database links:

Vimentin抗体溶解方法：

1、在我们公司购买抗体后，查看标签上的规格，如：0.1ml、0.2ml

2、 以规格为0.1ml的冻干粉抗体为例（部分抗体为液体，无需再溶解）：

溶解方法一：用移液器加0.1ml双蒸水到试管内，混匀，-20℃保存；

若为其它规格（如：0.2ml）的冻干粉抗体，则加与标签上规格一致的（如：0.2ml）双蒸水溶解，混匀，-20℃保存。

溶解方法二：用移液器加 0.05ml（规格的 50%）双蒸水到试管内，再加 0.05ml（规格的 50%）

的甘油到试管内，混匀，-20℃保存； 若为其它规格（如：0.2ml）的冻干粉抗体，则加标签上规格的 50%（如：0.1ml）双蒸水，再加标签上规格的50%（如：0.1ml）甘油溶解，混匀，-20℃保存。

3、抗体的分装

抗体溶解后，将其分装成 5-10支（以0.1mlVimentin抗体为例，20ulx5支或 10ulx10支），-20℃保存，用一支取一支，避免反复冻溶。

另：预试验可做几个梯度1：100、1：200、1：400稀释，如果背景高，还可再稀释，选一个*佳的稀释比例，再正式做，效果*好。工作液可用“抗体稀释液”稀释。

后续试验时，再使用对应的缓冲液稀释成工作液，即用即配。

我们的抗体（冻干粉），在常温放置下，至少一个月有效；若在-20℃下保存（推荐），至少三年有效。

Vimentin抗体关联产品列表：

产品留言

标题

联系人

联系电话

内容

验证码

点击换一张

注：1.可以使用快捷键Alt+S或Ctrl+Enter发送信息!
2.如有必要,请您留下您的详细联系方式!