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产品编号bs-1011R
英文名称CXCR4
中文名称细胞表面趋化因子受体4抗体
别 名C-X-C chemokine receptor type 4; CXC-R4; CXCR-4; Stromal cell-derived factor 1 receptor; SDF-1 receptor; Fusin; Leukocyte-derived seven transmembrane domain receptor; LESTR; CD184 antigen; CXCR4_HUMAN.
JournalPMIDIFApplicationClinical Journal of the American Society of Nephrology (2013)242625098.9800IHC-PClinical Cancer Research (2012)229326667.8370neutralizingBMC Cancer (2014)249171863.3300WBJournal of Cellular Biochemistry (2015)263654323.2630WBInternational Journal of Molecular Sciences (2015)262518942.8620IHC-FJournal of Oral and Maxillofacial Surgery (2016)268513141.4250IHC-PJournal of Research in Medical Sciences (2013)243798390.6840IHC
规格价格50ul/780元 购买 100ul/1280元 购买 200ul/1980元 购买 大包装/询价
说 明 书50ul 100ul 200ul
研究领域肿瘤 **学 细胞膜受体 细胞类型标志物
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Cow, Rabbit,
CXCR4抗体产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
分 子 量177kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Nestin
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
CXCR4抗体产品介绍background:
Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.
Function:
Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Subunit:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3.
Subcellular Location:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, tis protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3
Tissue Specificity:
CNS stem cells.
Post-translational modifications:
Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
Similarity:
Belongs to the intermediate filament family.
SWISS:
P48681
Gene ID:
10763
Database links:
CXCR4抗体溶解方法：
1、在我们公司购买抗体后，查看标签上的规格，如：0.1ml、0.2ml
2、 以规格为0.1ml的冻干粉抗体为例（部分抗体为液体，无需再溶解）：
溶解方法一：用移液器加0.1ml双蒸水到试管内，混匀，-20℃保存；
若为其它规格（如：0.2ml）的冻干粉抗体，则加与标签上规格一致的（如：0.2ml）双蒸水溶解，混匀，-20℃保存。
溶解方法二：用移液器加 0.05ml（规格的 50%）双蒸水到试管内，再加 0.05ml（规格的 50%）
的甘油到试管内，混匀，-20℃保存； 若为其它规格（如：0.2ml）的冻干粉抗体，则加标签上规格的 50%（如：0.1ml）双蒸水，再加标签上规格的50%（如：0.1ml）甘油溶解，混匀，-20℃保存。
3、抗体的分装
抗体溶解后，将其分装成 5-10支（以0.1mlCXCR4抗体为例，20ulx5支或 10ulx10支），-20℃保存，用一支取一支，避免反复冻溶。
另：预试验可做几个梯度1：100、1：200、1：400稀释，如果背景高，还可再稀释，选一个*佳的稀释比例，再正式做，效果*好。工作液可用“抗体稀释液”稀释。
后续试验时，再使用对应的缓冲液稀释成工作液，即用即配。
我们的抗体（冻干粉），在常温放置下，至少一个月有效；若在-20℃下保存（推荐），至少三年有效。
CXCR4抗体关联产品列表：
bs-3026R No 合格 phospho-AMPK beta 1 (Ser108) 磷酸化腺苷单磷酸活化蛋白激酶β1抗体
bs-3028R No 合格 phospho-Amyloid Precursor Protein (Thr668) 磷酸化APP淀粉样肽前体蛋白抗体
bs-3031R YES 合格 phospho-ASK1 (Thr845) 磷酸化细胞凋亡信号调节激酶1抗体
bs-3039R No 合格 Phospho-Acetyl CoA Carboxylase(Ser79) 磷酸化乙酰辅酶A羧化酶1ACCα抗体
bs-3043R No 合格 phospho-Akt (Thr450) 磷酸化蛋白激酶B抗体
bs-3050R YES 合格 Phospho-Aurora A (Thr288) 磷酸化有丝分裂激酶A抗体
bs-3054R YES 合格 Phospho-BLNK (Tyr96) 磷酸化T**细胞连接蛋白抗体
bs-3063R No 合格 Phospho-CEBP beta (Thr235) 磷酸化转录调节因子CEBP β抗体
bs-3064R No 合格 Phospho-ATP1A1 (Ser23) 磷酸化钠钾ATP酶蛋白a1抗体
bs-3065R No 合格 Phospho-CD19 (Tyr531) 磷酸化CD19抗体
bs-3068R YES 合格 Phospho-CRMP2 (Thr514) 磷酸化二氢嘧啶酶样2抗体
bs-3079R No 合格 合格 phospho-MCSF Receptor(Tyr923) 磷酸化巨噬细胞集落刺激因子受体抗体
bs-3082R YES 合格 合格 合格 Phospho-Caspase-9 (Thr125) 磷酸化半胱氨酸蛋白酶9抗体
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