beta Amyloid 1-28抗体经ICC, IHC, WB, FACS严格验证,品质保证.广泛种属反应性附有用户反馈信息.确保特异性!**的技术支持帮您更快得到实验结果,现货.我司提供8万种科研用专业抗体,经严格质控,特异性高,可重复性强,验证数..可与小鼠, 大鼠, 人样本反应并经WB, IHC, ICC实验严格验证。所有产品均提供质保服务,中国75%以上现货。
产品编号bs-10429R
英文名称beta Amyloid 1-28
中文名称β淀粉样肽1-28/β-Amyloid 1-28抗体
别 名beta Amyloid(1-28); beta-amyloid peptide; beta-Amyloid 1-28; beta-Amyloid 1-28; Amyloid 1-28; A4; AAA; ABETA; ABPP; AD1; Alzheimers Disease Amyloid Protein; Amyloid B; Amyloid Beta A4 Protein Precursor; Amyloid Beta; Amyloid of Aging and Alzheimer Disease; APP; APPI; B Amyloid; Beta APP; Cerebral Vascular Amyloid Peptide; CTFgamma; CVAP; PN II; PN2; PreA4; Protease nexin II; A beta; A4_HUMAN.
规格价格100ul/1280元 购买 200ul/1980元 购买 大包装/询价
说 明 书100ul 200ul
研究领域细胞生物 神经生物学 信号转导 细胞凋亡 Alzheimer's
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Horse, Rabbit, Sheep,
beta Amyloid 1-28抗体产品应用ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量177kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Nestin
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
beta Amyloid 1-28抗体产品介绍background:
Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.
Function:
Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Subunit:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3.
Subcellular Location:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, tis protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3
Tissue Specificity:
CNS stem cells.
Post-translational modifications:
Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
Similarity:
Belongs to the intermediate filament family.
SWISS:
P48681
Gene ID:
10763
Database links:
beta Amyloid 1-28抗体溶解方法:
1、在我们公司购买抗体后,查看标签上的规格,如:0.1ml、0.2ml
2、 以规格为0.1ml的冻干粉抗体为例(部分抗体为液体,无需再溶解):
溶解方法一:用移液器加0.1ml双蒸水到试管内,混匀,-20℃保存;
若为其它规格(如:0.2ml)的冻干粉抗体,则加与标签上规格一致的(如:0.2ml)双蒸水溶解,混匀,-20℃保存。
溶解方法二:用移液器加 0.05ml(规格的 50%)双蒸水到试管内,再加 0.05ml(规格的 50%)
的甘油到试管内,混匀,-20℃保存; 若为其它规格(如:0.2ml)的冻干粉抗体,则加标签上规格的 50%(如:0.1ml)双蒸水,再加标签上规格的50%(如:0.1ml)甘油溶解,混匀,-20℃保存。
3、抗体的分装
抗体溶解后,将其分装成 5-10支(以0.1mlbeta Amyloid 1-28抗体为例,20ulx5支或 10ulx10支),-20℃保存,用一支取一支,避免反复冻溶。
另:预试验可做几个梯度1:100、1:200、1:400稀释,如果背景高,还可再稀释,选一个*佳的稀释比例,再正式做,效果*好。工作液可用“抗体稀释液”稀释。
后续试验时,再使用对应的缓冲液稀释成工作液,即用即配。
我们的抗体(冻干粉),在常温放置下,至少一个月有效;若在-20℃下保存(推荐),至少三年有效。
beta Amyloid 1-28抗体关联产品列表:
bs-7710R No 合格 CENPP 着丝粒蛋白P抗体
bs-7712R No 合格 Importin4 核蛋白4抗体(Ran结合蛋白4)
bs-7714R YES 合格 CDCA1 细胞分裂周期相关蛋白1抗体
bs-7721R No 合格 CAPG2 染色体相关蛋白2抗体
bs-7723R No 合格 REC8 减数分裂重组蛋白家族REC8抗体
bs-7734R No 合格 CAPD3 浓缩素2复合亚基D3抗体
bs-7738R No 合格 Anillin 胞环蛋白肌动蛋白结合蛋白抗体
bs-7746R No 合格 DCTN3 动力蛋白激活蛋白亚基3抗体
bs-7757R No 合格 NAT10 乙酰基转移酶10抗体
bs-7773R No 合格 SPECC1L 细胞质和纺锤体机化蛋白A抗体
bs-7777R No 合格 VPS4a 液泡分选蛋白4抗体
bs-7781R No 合格 CDC123 细胞分裂周期蛋白CDC123抗体
bs-7783R No 合格 CDC20B 细胞分裂周期蛋白20B抗体
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