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DYNC1I2抗体

DYNC1I2抗体
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  • 产品名称:DYNC1I2抗体
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  • 产品展商:Bioss
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简单介绍
DYNC1I2抗体经ICC, IHC, WB, FACS严格验证,品质保证.广泛种属反应性附有用户反馈信息.确保特异性!**的技术支持帮您更快得到实验结果,现货.​我司提供8万种科研用专业抗体,经��格质控,特异性高,可重复性强,验证数.DYNC1I2抗体可与小鼠, 大鼠, 人样本反应并经WB, IHC, ICC实验严格验证。所有产品均提供质保服务,中国75%以上现货。
产品描述

 

DYNC1I2抗体ICC, IHC, WB, FACS严格验证,品质保证.广泛种属反应性附有用户反馈信息.确保特异性!**的技术支持帮您更快得到实验结果,现货.我司提供8万种科研用专业抗体,经严格质控,特异性高,可重复性强,验证数..可与小鼠, 大鼠, 人样本反应并经WB, IHC, ICC实验严格验证。所有产品均提供质保服务,中国75%以上现货。

产品编号bs-10471R

英文名称DYNC1I2

中文名称胞浆动力蛋白中间链2抗体

别    名Cytoplasmic dynein 1 intermediate chain 2; Cytoplasmic dynein intermediate chain 2; Dynein intermediate chain 2, cytosolic; DH IC-2; DC1I2_HUMAN; DNCI2; DNCIC2; Dynein cytoplasmic intermediate polypeptide 2.  

规格价格100ul/1280元 购买    200ul/1980元 购买    大包装/询价

说 明 书100ul  200ul

研究领域细胞生物  神经生物学  信号转导  细胞粘附分子  细胞骨架  

抗体来源Rabbit

克隆类型Polyclonal

交叉反应 Human, Mouse, Rat, Dog, Pig, Horse, Rabbit, Sheep,

DYNC1I2抗体产品应用ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

177kDa

细胞定位细胞浆

   Lyophilized or Liquid

   1mg/1ml

KLH conjugated synthetic peptide derived from human Nestin

   IgG

纯化方法affinity purified by Protein A

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMedPubMed

DYNC1I2抗体产品介绍background:

Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.

Function:

Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.

Subunit:

Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3.

Subcellular Location:

Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, tis protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3

Tissue Specificity:

CNS stem cells.

Post-translational modifications:

Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.

Similarity:

Belongs to the intermediate filament family.

SWISS:

P48681

Gene ID:

10763

Database links:

DYNC1I2抗体溶解方法:

1、在我们公司购买抗体后,查看标签上的规格,如:0.1ml、0.2ml

2、 以规格为0.1ml的冻干粉抗体为例(部分抗体为液体,无需再溶解):

溶解方法一:用移液器加0.1ml双蒸水到试管内,混匀,-20℃保存;

若为其它规格(如:0.2ml)的冻干粉抗体,则加与标签上规格一致的(如:0.2ml)双蒸水溶解,混匀,-20℃保存。

溶解方法二:用移液器加 0.05ml(规格的 50%)双蒸水到试管内,再加 0.05ml(规格的 50%)

的甘油到试管内,混匀,-20℃保存; 若为其它规格(如:0.2ml)的冻干粉抗体,则加标签上规格的 50%(如:0.1ml)双蒸水,再加标签上规格的50%(如:0.1ml)甘油溶解,混匀,-20℃保存。

3、抗体的分装

抗体溶解后,将其分装成 5-10支(以0.1mlDYNC1I2抗体为例,20ulx5支或 10ulx10支),-20℃保存,用一支取一支,避免反复冻溶。

另:预试验可做几个梯度1:100、1:200、1:400稀释,如果背景高,还可再稀释,选一个*佳的稀释比例,再正式做,效果*好。工作液可用“抗体稀释液”稀释。

后续试验时,再使用对应的缓冲液稀释成工作液,即用即配。

我们的抗体(冻干粉),在常温放置下,至少一个月有效;若在-20℃下保存(推荐),至少三年有效。

DYNC1I2抗体关联产品列表:

bs-8878R YES 合格 LC3 自噬微管相关蛋白轻链3抗体
bs-8929R YES 合格 Cyclin E1 周期素E1抗体
bs-8987R No 合格 合格 Connexin 43 间隙连接蛋白43抗体
bs-8995R No 合格 合格 IFN-Alpha 干扰素α抗体
bs-8996R YES 合格 合格 CD34 CD34抗体
bs-8997R No 合格 合格 Annexin VI 膜粘连蛋白6抗体
bs-9005R No 合格 CECR1 猫眼综合征染色体候选基因1抗体
bs-9006R No 合格 CECR6 猫眼综合征染色体候选基因6抗体
bs-9015R No 合格 EFHD1 EFHD1蛋白抗体
bs-9022R No 合格 IQCG IQCG蛋白抗体
bs-9028R No 合格 AKAP2/PALM2 蛋白激酶A2抗体
bs-9048R No 合格 TRPM6 瞬时受体电位离子通道蛋白6抗体(M亚家族)
bs-9051R No 合格 TRPM4 瞬时受体电位离子通道蛋白4抗体(M亚家族)
bs-9052R No 合格 DIS3L 有丝分裂控制样蛋白DIS3L抗体
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