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phospho-KMT6抗体

phospho-KMT6抗体
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  • 产品名称:phospho-KMT6抗体
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  • 产品展商:Bioss
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简单介绍
phospho-KMT6抗体经ICC, IHC, WB, FACS严格验证,品质保证.广泛种属反应性附有用户反馈信息.确保特异性!**的技术支持帮您更快得到实验结果,现货.​我司提供8万种科研用专业抗体,经严格质控,特异性高,可重复性强,验证数.phospho-KMT6抗体可与小鼠, 大鼠, 人样本反应并经WB, IHC, ICC实验严格验证。所有产品均提供质保服务,中国75%以上现货。
产品描述

 

phospho-KMT6抗体ICC, IHC, WB, FACS严格验证,品质保证.广泛种属反应性附有用户反馈信息.确保特异性!**的技术支持帮您更快得到实验结果,现货.我司提供8万种科研用专业抗体,经严格质控,特异性高,可重复性强,验证数..可与小鼠, 大鼠, 人样本反应并经WB, IHC, ICC实验严格验证。所有产品均提供质保服务,中国75%以上现货。

产品编号bs-10495R

英文名称phospho-KMT6 (Ser21)

中文名称磷酸化抑癌蛋白EZH2抗体

别    名KMT6 / EZH2 (phospho S21); KMT6 / EZH2 (phospho S21); KMT6/EZH2 (phospho Ser21); p-KMT6/EZH2 (Ser21); p-KMT6/EZH2 (S21); Enhancer of zeste homolog 2; Enx1h; MGC9169; Enhancer of zeste 2; ENX-1; ENX 1; ENX1; EZH1; EZH2; EZH 2; EZH2_HUMAN; Histone-lysine N-methyltransferase EZH2; KMT6A; Lysine N-methyltransferase 6; Enhancer of zeste homolog 2 (Drosophila); Enhancer of zeste, Drosophila, homolog 2; KMT 6; KMT6; KMT6A; WVS2.  

规格价格100ul/1580元 购买        大包装/询价

说 明 书100ul  

产品类型磷酸化抗体 甲基化抗体

研究领域**学  微生物学  b-**细胞  

抗体来源Rabbit

克隆类型Polyclonal

交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, Guinea Pig, Xenopus laevis, Zebrafish,

phospho-KMT6抗体产品应用ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

177kDa

细胞定位细胞浆

   Lyophilized or Liquid

   1mg/1ml

KLH conjugated synthetic peptide derived from human Nestin

   IgG

纯化方法affinity purified by Protein A

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMedPubMed

phospho-KMT6抗体产品介绍background:

Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.

Function:

Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.

Subunit:

Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3.

Subcellular Location:

Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, tis protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3

Tissue Specificity:

CNS stem cells.

Post-translational modifications:

Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.

Similarity:

Belongs to the intermediate filament family.

SWISS:

P48681

Gene ID:

10763

Database links:

phospho-KMT6抗体溶解方法:

1、在我们公司购买抗体后,查看标签上的规格,如:0.1ml、0.2ml

2、 以规格为0.1ml的冻干粉抗体为例(部分抗体为液体,无需再溶解):

溶解方法一:用移液器加0.1ml双蒸水到试管内,混匀,-20℃保存;

若为其它规格(如:0.2ml)的冻干粉抗体,则加与标签上规格一致的(如:0.2ml)双蒸水溶解,混匀,-20℃保存。

溶解方法二:用移液器加 0.05ml(规格的 50%)双蒸水到试管内,再加 0.05ml(规格的 50%)

的甘油到试管内,混匀,-20℃保存; 若为其它规格(如:0.2ml)的冻干粉抗体,则加标签上规格的 50%(如:0.1ml)双蒸水,再加标签上规格的50%(如:0.1ml)甘油溶解,混匀,-20℃保存。

3、抗体的分装

抗体溶解后,将其分装成 5-10支(以0.1mlphospho-KMT6抗体为例,20ulx5支或 10ulx10支),-20℃保存,用一支取一支,避免反复冻溶。

另:预试验可做几个梯度1:100、1:200、1:400稀释,如果背景高,还可再稀释,选一个*佳的稀释比例,再正式做,效果*好。工作液可用“抗体稀释液”稀释。

后续试验时,再使用对应的缓冲液稀释成工作液,即用即配。

我们的抗体(冻干粉),在常温放置下,至少一个月有效;若在-20℃下保存(推荐),至少三年有效。

phospho-KMT6抗体关联产品列表:

bs-9473R No 合格 NFAT5 活化T细胞核因子5蛋白抗体
bs-9475R No 合格 CNN2 钙结合蛋白Calponin 2抗体
bs-9478R No 合格 合格 SARP1 分泌细胞凋亡相关蛋白1抗体
bs-9483R No 合格 C5orf35 5号染色体开放阅读框35抗体
bs-9484R No 合格 合格 C5orf51 5号染色体开放阅读框51抗体
bs-9493R No 合格 合格 C9ORF43 9号染色体开放阅读框43抗体
bs-9494R No 合格 C9orf114 9号染色体开放阅读框114抗体
bs-9497R No 合格 C9orf152 9号染色体开放阅读框152抗体
bs-9503R No 合格 Factor XIIa heavy chain 凝血因子12重链抗体
bs-9504R No 合格 Kininogen-1 light chain 高分子量激肽原轻链抗体
bs-9505R YES 合格 alpha 2 Macroglobulin α2巨球蛋白(α-2M)抗体
bs-9511R No 合格 Proteinase-activated receptor 4/PAR4 蛋白酶激活受体4抗体
bs-9514R No 合格 PI14 蛋白酶抑制剂14抗体
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