ADFP抗体经ICC, IHC, WB, FACS严格验证,品质保证.广泛种属反应性附有用户反馈信息.确保特异性!**的技术支持帮您更快得到实验结果，现货.我司提供8万种科研用专业抗体,经严格质控,特异性高,可重复性强,验证数..可与小鼠, 大鼠, 人样本反应并经WB, IHC, ICC实验严格验证。所有产品均提供质保服务,中国75%以上现货。
产品编号bs-1164R
英文名称ADFP
中文名称脂肪组织分化相关蛋白抗体
别 名ADFP; Adipophilin; Adipose differentiation related protein; Adipose differentiation-related protein; Perilipin-2; PLIN2; PLIN2_HUMAN; adipophilin; Adipose differentiation related protein; ADRP; MGC10598
JournalPMIDIFApplicationDiabetes (2015)258456638.4700IF
规格价格50ul/780元 购买 100ul/1280元 购买 200ul/1980元 购买 大包装/询价
说 明 书50ul 100ul 200ul
研究领域肿瘤 心血管 细胞生物 **学 信号转导 新陈代谢
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat,
ADFP抗体产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user
分 子 量177kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Nestin
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
ADFP抗体产品介绍background:
Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.
Function:
Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Subunit:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3.
Subcellular Location:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, tis protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3
Tissue Specificity:
CNS stem cells.
Post-translational modifications:
Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
Similarity:
Belongs to the intermediate filament family.
SWISS:
P48681
Gene ID:
10763
Database links:
ADFP抗体溶解方法：
1、在我们公司购买抗体后，查看标签上的规格，如：0.1ml、0.2ml
2、 以规格为0.1ml的冻干粉抗体为例（部分抗体为液体，无需再溶解）：
溶解方法一：用移液器加0.1ml双蒸水到试管内，混匀，-20℃保存；
若为其它规格（如：0.2ml）的冻干粉抗体，则加与标签上规格一致的（如：0.2ml）双蒸水溶解，混匀，-20℃保存。
溶解方法二：用移液器加 0.05ml（规格的 50%）双蒸水到试管内，再加 0.05ml（规格的 50%）
的甘油到试管内，混匀，-20℃保存； 若为其它规格（如：0.2ml）的冻干粉抗体，则加标签上规格的 50%（如：0.1ml）双蒸水，再加标签上规格的50%（如：0.1ml）甘油溶解，混匀，-20℃保存。
3、抗体的分装
抗体溶解后，将其分装成 5-10支（以0.1mlADFP抗体为例，20ulx5支或 10ulx10支），-20℃保存，用一支取一支，避免反复冻溶。
另：预试验可做几个梯度1：100、1：200、1：400稀释，如果背景高，还可再稀释，选一个*佳的稀释比例，再正式做，效果*好。工作液可用“抗体稀释液”稀释。
后续试验时，再使用对应的缓冲液稀释成工作液，即用即配。
我们的抗体（冻干粉），在常温放置下，至少一个月有效；若在-20℃下保存（推荐），至少三年有效。
ADFP抗体关联产品列表：
%BC%09%E5%90%88%E6%A0%BC%09%09phospho-CD19+(Ser227)%09%E7%A3%B7%E9%85%B8%E5%8C%96CD19%E6%8A%97%E4%BD%93%3Cbr+%2F%3E%0D%0Abs-4790R%09No%09%09%09%E5%90%88%E6%A0%BC%09CD8%09CD8%E6%8A%97%E4%BD%93%3Cbr+%2F%3E&ProductInfo.JoinDate=2017-09-27+15%3A49%3A48.666&ProductInfo.Manufacturer=BIOSS&ProductInfo.CustomID=100ul++200ul&ProductInfo.Inventory=50&ProductInfo.Price=1280&ProductInfo.DiscountPrice=0.00&ProductInfo.MemberPrice=0.00&ProductInfo.EntryName=%E8%B7%A8%E8%86%9C%E8%9B%8B%E7%99%BD59%E6%A0%B7%E8%9B%8B%E7%99%BD%E6%8A%97%E4%BD%93&CommendType=1&ProductInfo.ProductID=0&ProductInfo.SmallImgName=2017927-155332201.png&ProductInfo.BigImgName=2017927-155332201.png&ProductInfo.MinImgUrl=http%3A%2F%2Fy1.yzimgs.com%2Fuploads%2F508782%2F2017927-155332201.png%3FimageView2%2F2%2Fw%2F200%2Fh%2F200%7Cwatermark%2F2%2Ftext%2F5LiK5rW35L-h6KOV55Sf54mp5oqA5pyv5pyJ6ZmQ5YWs5Y-4%2Ffont%2F5a6L5L2T%2Ffontsize%2F300%2Ffill%2FI0E3QTlBOA%3D%3D%2Fgravity%2FSouthEast&ProductInfo.MidImgUrl=http%3A%2F%2Fy1.yzimgs.com%2Fuploads%2F508782%2F2017927-155332201.png%3FimageView2%2F2%2Fw%2F200%2Fh%2F200%7Cwatermark%2F2%2Ftext%2F5LiK5rW35L-h6KOV55Sf54mp5oqA5pyv5pyJ6ZmQ5YWs5Y-4%2Ffont%2F5a6L5L2T%2Ffontsize%2F300%2Ffill%2FI0E3QTlBOA%3D%3D%2Fgravity%2FSouthEast&ProductInfo.MaxImgUrl=http%3A%2F%2Fy1.yzimgs.com%2Fuploads%2F508782%2F2017927-155332201.png%3Fwatermark%2F2%2Ftext%2F5LiK5rW35L-h6KOV55Sf54mp5oqA5pyv5pyJ6ZmQ5YWs5Y-4%2Ffont%2F5a6L5L2T%2Ffontsize%2F300%2Ffill%2FI0E3QTlBOA%3D%3D%2Fgravity%2FSouthEast&CheckDataIntro=true&CheckContent=true&X-Requested-With=XMLHttpRequestun, ����; font-size: 12px; word-wrap: break-word; word-break: keep-all;">not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end usebs-4791R YES 合格 合格 CD8 alpha CD8抗体
bs-4817R No 合格 CD4 CD4抗体
bs-4818R YES 合格 CD45 白细胞共同抗原抗体
bs-4819R YES 合格 CD45 白细胞共同抗原抗体
bs-4820R No 合格 CD45 白细胞共同抗原抗体
bs-4821R No 合格 AFP 甲胎蛋白AFP抗体
bs-4826R No 合格 CD83 CD83抗体
bs-4827R No 合格 FHL1 骨骼肌蛋白FHL1抗体
bs-4834R YES 合格 CD73 CD73抗体
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