phospho-SAPK3抗体经ICC, IHC, WB, FACS严格验证,品质保证.广泛种属反应性附有用户反馈信息.确保特异性!**的技术支持帮您更快得到实验结果，现货.我司提供8万种科研用专业抗体,经严格质控,特异性高,可重复性强,验证数..可与小鼠, 大鼠, 人样本反应并经WB, IHC, ICC实验严格验证。所有产品均提供质保服务,中国75%以上现货。
产品编号bs-12001R
英文名称phospho-SAPK3 (Thr183+Tyr185)
中文名称磷酸化丝裂原活化蛋白激酶磷酸酶p38γ抗体
别 名p-SAPK3(phospho-Thr183+Tyr185); MAP kinase 12; MAP kinase p38 gamma; MAPK 12; Mapk12; Mitogen Activated Protein Kinase 12; ERK6; ERK 6; ERK-6; Extracellular signal-regulated kinase 6; Mitogen activated protein kinase 3; Mitogen activated protein kinase p38 gamma; Mitogen-activated protein kinase 12; Mitogen-activated protein kinase p38 gamma; MK12_HUMAN; P38 GAMMA; P38GAMMA; PRKM12; SAPK 3; SAPK3; Stress Activated Protein Kinase 3; Stress-activated protein kinase 3.
规格价格100ul/1580元 购买 大包装/询价
说 明 书100ul
产品类型磷酸化抗体
研究领域细胞生物 信号转导 细胞凋亡 激酶和磷酸酶 细胞粘附分子 细胞分化
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit, Guinea Pig,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量177kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Nestin
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
phospho-SAPK3抗体产品介绍background:
Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.
Function:
Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Subunit:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3.
Subcellular Location:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, tis protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3
Tissue Specificity:
CNS stem cells.
Post-translational modifications:
Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
Similarity:
Belongs to the intermediate filament family.
SWISS:
P48681
Gene ID:
10763
Database links:
phospho-SAPK3抗体溶解方法：
1、在我们公司购买抗体后，查看标签上的规格，如：0.1ml、0.2ml
2、 以规格为0.1ml的冻干粉抗体为例（部分抗体为液体，无需再溶解）：
溶解方法一：用移液器加0.1ml双蒸水到试管内，混匀，-20℃保存；
若为其它规格（如：0.2ml）的冻干粉抗体，则加与标签上规格一致的（如：0.2ml）双蒸水溶解，混匀，-20℃保存。
溶解方法二：用移液器加 0.05ml（规格的 50%）双蒸水到试管内，再加 0.05ml（规格的 50%）
的甘油到试管内，混匀，-20℃保存； 若为其它规格（如：0.2ml）的冻干粉抗体，则加标签上规格的 50%（如：0.1ml）双蒸水，再加标签上规格的50%（如：0.1ml）甘油溶解，混匀，-20℃保存。
3、抗体的分装
抗体溶解后，将其分装成 5-10支（以0.1mlphospho-SAPK3抗体为例，20ulx5支或 10ulx10支），-20℃保存，用一支取一支，避免反复冻溶。
另：预试验可做几个梯度1：100、1：200、1：400稀释，如果背景高，还可再稀释，选一个*佳的稀释比例，再正式做，效果*好。工作液可用“抗体稀释液”稀释。
后续试验时，再使用对应的缓冲液稀释成工作液，即用即配。
我们的抗体（冻干粉），在常温放置下，至少一个月有效；若在-20℃下保存（推荐），至少三年有效。
phospho-SAPK3抗体关联产品列表：
bs-0516R No 合格 RAR Beta 维甲酸受体β抗体
bs-0521R YES 合格 CD44 CD44抗体
bs-0522R YES 合格 CD45 白细胞共同抗原CD45抗体
bs-0523R YES 合格 合格 SOX2 胚胎干细胞关键蛋白抗体
bs-0526R YES 合格 ALOX5/5 lipoxygenase 5-脂氧合酶抗体
bs-0527R No 合格 合格 CD10 CD10抗体
bs-0528R YES 合格 CD24 CD24抗体
bs-0529R No 合格 IL-18 白细胞介素-18/干扰素γ诱导因子抗体
bs-0530R No 合格 合格 PPAR gamma 过氧化酶活化增生受体γ抗体
bs-0531R No 合格 合格 Matriptase 蛋白裂解酶（一种新的癌基因）抗体
bs-0535R No 合格 合格 TFF3 三叶肽因子3抗体
bs-0536R No 合格 Interferon alpha 2b 干扰素α2b抗体
bs-0537R No 合格 ANGPTL2 血管生成素样蛋白2抗体
bs-0541R No 合格 CAMK2A + CAMK2B + CAMK2D 钙/钙调素依赖蛋白激酶2b/2γ抗体
bs-0542R YES 合格 CDK1 周期素依赖性激酶1抗体
bs-0545R YES 合格 合格 SCF 干细胞生长因子抗体
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