英文名称APOJ
中文名称载脂蛋白J抗体
别 名AAG 4; AAG4 ;Aging associated protein 4; Clusterin-alpha/beta; APOJ; Apo J; Apolipoprotein J; Cli; CLU; Clusterin alpha chain; Clusterin; Clusterin beta chain; Complement associated protein SP 40 40; Complement associated protein SP 40; Complement associated protein SP40; Complement cytolysis inhibitor; Complement lysis inhibitor; KUB 1; KUB1; MGC24903; NA1/NA2; SGP 2; SGP2; SP 40; SP40; Sulfated glycoprotein 2; Testosterone repressed prostate message 2; TRPM 2; TRPM2; Clusterin- Alpha/Beta; Apolipoprotein J.
JournalPMIDIFApplicationJournal of Molecular Neuroscience (2014)244885742.3730IHC-P
说 明 书50ul 100ul 200ul
研究领域肿瘤 心血管 细胞生物 **学 脂蛋白
抗体来源Rabbit
克隆类型Polyclonal
APOJ抗体交叉反应 Human, Mouse, Rat,
产品应用ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量50kDa
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human APO J
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
Apolipoprotein J is the major secreted product of Sertoli cells and is thought to play a critical role in spermatogenesis. The protein was shown to be a normal constituent of human blood and is also thought to be a control mechanism of the complement cascade. It prevents the binding of a C5b-C7 complex to the membrane of the target cell and in this way inhibits complement-mediated cytolysis.
Apolipoprotein J is overexpressed in human prostate and breast cancers and in squamous cell carcinomas. Suppression of Apolipoprotein J renders these cells sensitive to chemotherapeutic drug-mediated apoptosis. It has been proposed that elevated levels of Apolipoprotein J may promote oncogenic transformation and tumor progression by interfering with BAX proapoptotic activities.
Function:
Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.
Subcellular Location:
Nucleus. Cytoplasm. Mitochondrion membrane. Cytoplasm, cytosol. Microsome. Endoplasmic reticulum. Cytoplasmic vesicle, secretory vesicle, chromaffin granule. Isoforms lacking the N-terminal signal sequence have been shown to be cytoplasmic and/or nuclear. Secreted isoforms can retrotranslocate from the secretory compartments to the cytosol upon cellular stress. Detected in perinuclear foci that may be aggresomes containing misfolded, ubiquitinated proteins. Detected at the mitochondrion membrane upon induction of apoptosis and Secreted. Can retrotranslocate from the secretory compartments to the cytosol upon cellular stress.
Tissue Specificity:
Detected in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Detected in the germinal center of colon lymphoid nodules and in colon parasympathetic ganglia of the Auerbach plexus (at protein level). Ubiquitous. Detected in brain, testis, ovary, liver and pancreas, and at lower levels in kidney, heart, spleen and lung.
Post-translational modifications:
Isoform 1 is proteolytically cleaved on its way through the secretory system, probably within the Golgi lumen. Polyubiquitinated, leading to proteasomal degradation.
Heavily N-glycosylated. About 30% of the protein mass is comprised of complex N-linked carbohydrate.
Similarity:
Belongs to the clusterin family.
SWISS:
P10909
Gene ID:
1191