TFEB抗体

TFEB抗体经ICC, IHC严格验证,品质保证.适用于多种种属反应性,被多篇文献引用并有用户反馈信息.确保特异性!产品具有以下特点:

1、全：网络全世界的上等产品，基本上各种抗体产品在该公司均能找到

2、新：产品及网站更新非常快，基本上每周均有新产品出现

3、优：产品质量好，投诉比较少

4、强：强大的技术支持队伍和力量，网站上有齐全的技术资料以及客户评论，并提供实时在线技术咨询，使您使用产品时没有任何后顾之忧。

产品编号xy-5137R

英文名称TFEB

中文名称T**细胞转录调节因子TFEB抗体

别    名Alpha TFEB; AlphaTFEB; bHLHe35; T cell transcription factor EB; T-cell transcription factor EB; TCFEB; Transcription factor EB; TFEB_HUMAN.  

JournalPMIDIFApplicationOncology Reports (2015)263527602.3010IHC-P

说 明 书50ul  100ul  200ul

研究领域细胞生物  **学  转录调节因子  t-**细胞  

抗体来源Rabbit

克隆类型Polyclonal

TFEB抗体交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit,

产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量52kDa

细胞定位细胞核 细胞浆

性    状Lyophilized or Liquid

浓    度1mg/1ml

免 疫 原KLH conjugated synthetic peptide derived from human TFEB

亚    型IgG

纯化方法affinity purified by Protein A

储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMedPubMed

产品介绍background:

Transcription factors of the basic helix-loop-helix zipper (bHLH-ZIP) family contain a basic domain, used for DNA binding, and HLH and ZIP domains, both used for oligomerization. TFEB was isolated from a human B-cell cDNA library using a binding sequence from the adenovirus major late promoter. This transcription factor probably binds to the USF/MLTF site and probably recognizes E-box sequences in the heavy-chain immunoglobulin enhancer.

Function:

Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta.

Subunit:

Homodimer and heterodimer; with TFE3 or MITF.

Subcellular Location:

Cytoplasm. Nucleus. Note=Mainly present in the cytoplasm. Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus.

Post-translational modifications:

Sumoylated; does not affect dimerization with MITF.

Similarity:

Belongs to the MiT/TFE family.

Contains 1 basic helix-loop-helix (bHLH) domain.

SWISS:

P19484

Gene ID:

7942